PKM2 (PKM) (Isoform M1) Rabbit Polyclonal Antibody

CAT#: AP26075PU-N

PKM2 (PKM) (Isoform M1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from U87 cells using PKM1 Antibody.

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  Immunofluorescence staining of methanol-fixed MEF cells using PKM1 Antibody.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western blot: 1/1000.
  Immunofluorescence.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around aa. 399~403 derived from Human PKM1.
• Specificity: This antibody detects endogenous levels of total PKM1 protein.
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity-chromatography using epitope-specific peptide
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 60 kDa
• Gene Name: pyruvate kinase, muscle
• Database Link:
  Entrez Gene 18746 MouseEntrez Gene 5315 Human
  UniProt ID P14618
• Background: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
• Synonyms: PK2, PK3, PKM, CTHBP, M2-PK, THBP1, OIP3, OIP-3, Pyruvate kinase 2/3, Pyruvate kinase M1/M2, Pyruvate kinase muscle

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways, Purine metabolism, Pyruvate metabolism, Type II diabetes mellitus