KRIT1 Rabbit Polyclonal Antibody

SKU AP26021PU-L

KRIT1 rabbit polyclonal antibody, Purified

Product Data

• Application: IF, WB
• Recommended Dilution: Western Blot: 1-5 μg/ml. ; Immunofluorescence: 1-10 μg/ml.; Immunohistochemistry: 1/200.
• Reactivity: Human
• Antibody Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Highly pure (>95%) recombinant Human CCM-1 (Met1-Ser736) derived from E. coli fused to a C-teminal His-tag (6 x His) (Cat.-No AR26002PU-N).
• Specificity: This antibody is anti-His depleated.; It detects KRIT1 / CCM1.
• Buffer: 5mM PBS, pH 7.2; State: Purified; State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.; Centrifuge vial prior to opening.
• Purification: Protein A Chromatography
• Conjugation: Unconjugated
• Storage:

  Prior to reconstitution store at 2-8°C.; Following reconstitution store undiluted at 2-8°C for one month; or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Shipping: Ambient
• Gene Name: KRIT1, ankyrin repeat containing
• Database Link:
  O00522
• Background: Cerebral Cavernous Malformations (CCM) are frequent vascular abnormalities caused by mutations in one of the CCM genes. CCM-1 (also known as KRIT1) stabilizes endothelial junctions and is essential for vascular morphogenesis in mouse embryos. However, cellular functions of CCM-1 during the early steps of the CCM pathogenesis remain unknown. It was shown that CCM-1 represents an antiangiogenic protein to keep the human endothelium quiescent. CCM-1 inhibits endothelial proliferation, apoptosis, migration, lumen formation, and sprouting angiogenesis in primary human endothelial cells. CCM-1 strongly induces DLL4-NOTCH signaling, which promotes AKT phosphorylation but reduces phosphorylation of the mitogen-activated protein kinase ERK. Consistently, blocking of NOTCH activity alleviates CCM-1 effects. ERK phosphorylation is increased in human CCM lesions. Transplantation of CCM-1-silenced human endothelial cells into SCID mice recapitulates hallmarks of the CCM pathology and serves as a unique CCM model system.
• Synonyms: Krev interaction trapped 1

Reference Data

• Protein Families: Druggable Genome