Lamin B1 (LMNB1) (C-term) Rabbit Polyclonal Antibody

SKU AP23277PU-N

Lamin B1 (LMNB1) (C-term) rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: IF, IHC, IP, WB
• Recommended Dilution: Western blot: At 1-2μg/ml with the appropriate system to detect Lamin B in cells and tissues.; Immunohistochemistry on paraffin sections: At 0.5-1μg/ml to detect Lamin B in formalin fixed and paraffin embedded tissues. Boiling the sections is required.; Immunohistochemistry in frozen sections: At 0.5-1μg/ml to detect Lamin B in formalin or acetone fixed tissues.; Immunocytochemistry: At 0.5-1μg/ml to detect Lamin B in acetone fixed cell. Antigen retrieval by Pepsin and Trypsin is required.; Immunoprecipitation.
• Reactivity: Human, Mouse, Rat
• Antibody Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a sequence at the C-terminal of human Lamin B
• Specificity: This antibody detects Lamin Beta at C-term. No cross reactivity with other proteins.
• Buffer: 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3; State: Aff - Purified; State: Lyophilized Ig fraction
• Reconstitution Method: 0.2 ml of distilled water will yield a concentration of 0.5 mg/ml.
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Ambient
• Gene Name: lamin B1
• Database Link:
  P20700
• Background: Lamins are the major components of the nuclear lamina which underlies the nuclear envelope of eukaryotic cells. lamin B is a structural component of the long-sought-after spindle matrix that promotes microtubule assembly and organization in mitosis. Inspection of the deduced amino acid sequence of lamin B revealed the presence in coil 1B of the alpha-helical domain of a leucine heptad repeat region.Lamin B assembled into a matrix-like network in mitosis through a process that depended on the presence of the guanosine triphosphate-bound form of the small guanosine triphosphatase Ran.
• Synonyms: Lamin B1/B2, LMN2, LMNB, Lamin B1, Lamin B2

Reference Data