Pou5f1 Goat Polyclonal Antibody

SKU AP22409PU-N

Pou5f1 goat polyclonal antibody, Aff - Purified

Product Data

• Application: ELISA, WB
• Recommended Dilution: Peptide ELISA: Limit Dlution: 1/16000.; Western blot: 1-2 µg/ml. Approx 38-40kDa band observed in lysates of cell line NIH3T3 and approx. 45kDa in lysates of cell line HepG2 (calculated MW of 38.6kDa according to Human NP_002692.2 and 38.2kDa according to Mouse NP_038661.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources.
• Reactivity: Human, Mouse, Rat
• Antibody Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_038661.2.
• Specificity: Recognizes OCT4/POU5F1.
• Buffer: Tris saline, pH~7.3 with 0.02% Sodium Azide and 0.5% BSA; State: Aff - Purified; State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: POU domain, class 5, transcription factor 1
• Database Link:
  P20263
• Background: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.; The POU-specific domain mediates interaction with PKM2 and is Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
• Synonyms: POU5F1, OCT3, OCT4, OTF3, POU domain, class 5, transcription factor 1, Octamer-binding transcription factor 3, Oct-3, Oct-4

Reference Data