ALDH5A1 (485-496) Goat Polyclonal Antibody

CAT#: AP22390PU-N

ALDH5A1 (485-496) goat polyclonal antibody, Aff - Purified

Product Images

ALDH5A1 antibody staining of Human Liver lysate at 0.5 ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  ALDH5A1 antibody staining of paraffin embedded Human Liver at 5 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Peptide ELISA: 1/32000 (Detection Limit).
  Western blot: 0.3-1 µg/ml. This antibody detects a band of Approx 55kDa in Human Liver lysates and 50kDa band in Mouse and Rat Liver lysates.
  Immunohistochemistry on Paraffin Sections: 5-10 µg/ml. In paraffin embedded Human Liver shows heavy textured cystoplasm staining in hepatocytes, consistent with mitochondia.
• Reactivities: Bovine, Canine, Human, Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of Human ALDH5A1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_733936.1; NP_001071.1).
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: aldehyde dehydrogenase 5 family member A1
• Database Link:
  Entrez Gene 214579 MouseEntrez Gene 291133 RatEntrez Gene 7915 Human
  UniProt ID P51649
• Background: Defects in ALDH5A1 are the cause of succinate semialdehyde dehydrogenase deficiency (SSADH deficiency) [MIM:271980]. SSADH deficiency is a rare inborn error in the metabolism of 4-aminobutyric acid (GABA) which leads to accumulation of 4-hydroxybutyric acid in physiologic fluids of patients. The disease is characterized by severe ataxia and by mildly retarded psychomotor development.
• Synonyms: SSADH; SSDH
• Note: Calculated Molecular Weight: 58.7kDa (NP_733936.1).

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Butanoate metabolism, Metabolic pathways