Stem bromelain Rabbit Polyclonal Antibody
Product Data | |
Application | ELISA, ID, IF, IP, R, WB |
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Recommended Dilution | This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques). Working Dilutions in non-precipitating antibody-binding techniques: 1/5,000-1/50,000. |
Antibody Host | Rabbit |
Isotype | IgG |
Clonality | Polyclonal |
Immunogen | Bromelain isolated and purified from Pineapple stem. Freund's complete adjuvant is used in the first step of the immunization procedure. |
Specificity | This antibody recognizes Bromelain from Pineapple stem. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined. |
Buffer | PBS, pH 7.2 without preservatives and foreign proteins Label: Biotin State: Lyophilized hyperimmune IgG fraction Molar radio: Biotin/IgG ~6.2 |
Concentration | lot specific |
Purification | Ammonium Sulphate Precipitation and Ion Exchange Chromatography |
Conjugation | Biotin |
Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. If a slight precipitation occurs upon storage, this should be removed by centrifugation. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Shipping | Ambient |
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