PRAK (MAPKAPK5) pSer93 Rabbit Polyclonal Antibody

SKU AP12637PU-N

PRAK (MAPKAPK5) pSer93 rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.; Western Blot: 1/100-1/500.; Immunohistochemistry: 1/50-1/100.
• Reactivity: Human, Mouse
• Antibody Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S93 of Human MAPKAPK.
• Specificity: This antibody detects MAPKAP Kinase-5 (MAPKAPK5) pSer93.
• Buffer: PBS with 0.09% (W/V) Sodium Azide as preservative.; State: Aff - Purified; State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: mitogen-activated protein kinase-activated protein kinase 5
• Database Link:
  Q8IW41
• Background: The protein encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites.
• Synonyms: MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAPK5, PRAK
• Note: Calculated Molecular weight: 54220 Da

Reference Data