LIN28B Rabbit Polyclonal Antibody

SKU AP11496PU-N

LIN28B rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: FC, IF, IHC, WB
• Recommended Dilution: Western blot: 1/1000. ; Flow Cytometry: 1/10-1/50. ; Immunofluorescence: 1/10-1/50.; Immunohistochemistry on Paraffin Sections: 1/10-1/50.
• Reactivity: Human, Mouse
• Antibody Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 95-128 amino acids from the Central region of Human LIN28B.
• Specificity: This antibody detects LIN28B at center.
• Buffer: PBS; State: Aff - Purified; State: Liquid purified Ig fraction; Preservative: 0.09% (W/V) Sodium Azide
• Concentration: lot specific
• Purification: Purified by Protein A column followed by peptide affinity purifcation.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Predicted Protein Size: 26953 Da
• Gene Name: lin-28 homolog B
• Database Link:
  Q6ZN17
• Background: Lin-28 homolog B (LIN28B) is overexpressed in hepatocellular carcinoma. The heterochronic gene lin-28 is a key regulator of developmental timing in the nematode Caenorhabditis elegans. Similar with lin-28 proteins, LIN28B conserves a cold shock domain and a pair of CCHC zinc finger domains. Phylogenetic analysis suggests that they might arise as a result of duplication from an ancestral gene. Overexpression of LIN28B was noted in most HCC cell lines and clinical samples. A short LIN28B isoform was also identified in non-tumor liver tissue and fetal liver. Although predominantly localized in the cytoplasm, LIN28B protein shows cell cycle-dependent nuclear translocation in Huh7 cells. Induced expression of exogenous LIN28B in a tet-off cell line promoted cancer cell proliferation.
• Synonyms: LIN28B, CSDD2, Lin-28 homolog B

Reference Data