Pk (V5) Epitope Tag (GKPIPNPLLGLDST) Rabbit Polyclonal Antibody
| Product Data | |
| Application | WB |
|---|---|
| Recommended Dilution | ELISA: 1/1000. Western Blot: ~~1/1000. |
| Antibody Host | Rabbit |
| Isotype | Ig |
| Clonality | Polyclonal |
| Immunogen | KLH conjugated synthetic peptide encoding the V5 tag (GKPIPNPLLGLDST) was used as antigen. |
| Buffer | PBS containing 0.09% (W/V) Sodium Azide as preservative. State: Purified State: Liquid purified Ig fraction. |
| Concentration | lot specific |
| Purification | Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Background | Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The V5 epitope tag is is composed of a 14 residue peptide, GKPIPNPLLGLDST, derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). This polyclonal antibody provides a simple solution to detect the expression of a V5-tagged protein in cells. |
| Reference Data | |
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