RAD54 (RAD54L) (1-17) Rabbit Polyclonal Antibody

SKU AP09254PU-N

RAD54 (RAD54L) (1-17) rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: ELISA, WB
• Recommended Dilution: Suitable for use in ELISA and by Western blot.; Expect a band approximately 84 kDa in size corresponding to RAD54 protein by Western blotting in the appropriate cell lysate or extract. Splice variants exist for this protein that may result in the detection of lower molecular weight bands.; Recommended Dilutions:; ELISA: 1/10000-1/40000.; Western Blot: 1/500-1/2000.
• Reactivity: Human
• Antibody Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding aa 1-17 of Human RAD54 protein.
• Specificity: BLAST analysis indicates 100% homology of the immunizing sequence with RAD54 from Human.; Cross reactivity with RAD54 protein homologues from other sources may not occur as sequence homology varies by at least one amino acid residue in this sequence.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide as preservative.; State: Aff - Purified; State: Liquid (sterile filtered) purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using peptide coupled to agarose beads.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: RAD54-like (S. cerevisiae)
• Database Link:
  Q92698
• Background: RAD54, also known as hHR54, HR54, hRAD54 and RAD54A, belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA pairing, and stimulate DNA recombination.
• Synonyms: RAD54A, RAD54 homolog, hRAD54, hHR54

Reference Data