VSV-g Epitope Tag (YTDIEMNRLGK) Rabbit Polyclonal Antibody
Product Data | |
Application | ELISA, IF, IHC, WB |
---|---|
Recommended Dilution | ELISA: 1:8,000 - 1:32,000. Western blot: 1:200 - 1:2,000. Immunoflourescence: 1:200 - 1:500. Immunohistochemistry:1:200 - 1:500. |
Antibody Host | Rabbit |
Isotype | IgG |
Clonality | Polyclonal |
Immunogen | Synthetic peptide corresponding aa 501-511 of vesicular stomatitis virus glycoprotein (VSV-G) |
Specificity | This affinity purified antibody is directed against VSV-G protein |
Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide State: Aff - Purified State: Liquid sterile filtered Ig fraction |
Concentration | lot specific |
Purification | Immunoaffinity chromatography |
Conjugation | Unconjugated |
Storage | Store the antibody at 2 - 8 °C up to one month or for longer (in aliquots) at -20 °C or below. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use. |
Stability | Shelf life: one year from despatch. |
Shipping | Blue Ice |
Background | Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. |
Reference Data |
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