L1 / ORF2 Chicken Polyclonal Antibody
| Product Data | |
| Application | ELISA, WB |
|---|---|
| Recommended Dilution | ELISA: 1/10000. Western Blot: 1/2000-1/10000. Expect a band approximately 149 kDa in size corresponding to L1 protein by western blotting in the appropriate cell lysate or extract. |
| Reactivity | Chimpanzee, Human |
| Antibody Host | Chicken |
| Isotype | IgY |
| Clonality | Polyclonal |
| Immunogen | Two Synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein |
| Specificity | This antibody recognizes L1 / ORF2. Reactivity occurs against Human L1/ORF2 protein and is useful in determining its presence in various assays. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 State: Ig Fraction State: Liquid IgY fraction Preservative: 0.01% (w/v) Sodium Azide |
| Concentration | lot specific |
| Purification | IgY fraction antibody purified from monospecific Chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer. |
| Conjugation | Unconjugated |
| Storage | Store the antibody at -20°C. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch |
| Shipping | Blue Ice |
| Background | The L1 (LINE-1), or Long INterspersed Element, retrotransposon ORF2 is the most common open reading frame in the human genome, present in various forms in many thousands of copies. This large family of proteins includes magnesium dependent endonucleases and a large number of phosphatases involved in intracellular signaling. Both intact ORF1 and ORF2 are absolutely required for autonomous retrotransposition. ORF2 encodes an endonuclease, reverse transcriptase, and zinc knuckle domains. The expression of ORF2 appears to be tightly regulated except in germ line tissues, embryonic tissues, and certain cancers including teratomas, testicular cancers, and leukemias. This antibody is intended for use in studying control of L1 retrotransposons. |
| Synonyms | LINE-1 |
| Reference Data | |
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