SYK Rabbit Polyclonal Antibody

SKU AP08103PU-S

SYK rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500~1/1000.; Immunofluorescence: 1/100~1/200.; Immunohistochemistry on Paraffin Sections: 1/50~1/100.
• Reactivity: Human
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequenece around amino acids 321~325 (N-P-Y-E-P) derived from Human Syk.
• Specificity: This antibody detects endogenous levels of total Syk protein.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.; State: Aff - Purified; State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.; Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Shipping: Blue Ice
• Gene Name: spleen tyrosine kinase
• Database Link:
  P43405
• Background: Syk (72 kDa) is a non receptor protein tyrosine kinase that plays an important role in immune receptor signal transduction and is implicated in endothelial cell functions, including cell growth and migration.; SYK is a positive effector of BCR stimulated responses. It couples the B cell antigen receptor (BCR) to the mobilization of calcium ions either through a phosphoinositide 3 kinase dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C gamma dependent pathway, when phosphorylated on Tyr 342 and Tyr 346. Thus the differential phosphorylation of SYK can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ions.
• Synonyms: Tyrosine-protein kinase SYK, Spleen tyrosine kinase

Reference Data