Nestin (NES) (1484-1500) Rabbit Polyclonal Antibody

SKU AP07829PU-N

Nestin (NES) (1484-1500) rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/20000 - 1/80000.; Immunofluorescence.; Immunohistochemistry on Paraffin Sections: 5 µg/ml.; Western Blot: 1/500 - 1/3000.
• Reactivity: Human, Mouse
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant protein corresponding to amino acids 1484-1500 of Human Nestin
• Specificity: This antibody recognizes Nestin.
• Buffer: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2; State: Aff - Purified; State: Liquid purified Ig fraction; Preservative: 0.01% (w/v) Sodium Azide
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch
• Shipping: Blue Ice
• Gene Name: nestin
• Database Link:
  P48681
• Background: Nestin, is a large intermediate filament protein (class Type VI) that was first identified with a monoclonal antibody by Hockfield and McKay (1985). Nestin is expressed predominantly in stem cells of the central nervous system in the neural tube. Nestin is expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.
• Synonyms: NES, Nbla00170

Reference Data