hnRNP L (HNRNPL) Rabbit Polyclonal Antibody

SKU AP06669PU-N

hnRNP L (HNRNPL) rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.; Immunohistochemistry on paraffin sections: 1/50-1/200.; Immunofluorescence: 1/50-1/200.
• Reactivity: Human, Mouse
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 30-80 of Human hnRNP L.
• Specificity: This antibody detects endogenous levels of hnRNP L protein.; (region surrounding Gly58)
• Buffer: Phosphate buffered saline (PBS), pH 7.2.; State: Aff - Purified; State: Liquid purified Ig fraction; Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Predicted Protein Size: ~ 60 kDa
• Gene Name: heterogeneous nuclear ribonucleoprotein L
• Database Link:
  P14866
• Background: Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription, pre-mRNA processing and mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II). There are approximately 20 known hnRNP proteins, which range in size from 34 kDa to 120 kDa, and their complexes are the majorconstituents of the spliceosome. The majority of hnRNP proteins components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm. hnRNP I, also designated polypyrimidine tract-binding protein (PTB) and its homolog hnRNP L bind to the 3' end of introns to modulate alternative splicing mechanisms of pre-mRNAs in normal cells and the translation of several viruses, including hepatitis C virus (HCV). The human hnRNP I gene maps to chromosome 19p13.3 and encodes a 57 kDa protein that is localized in the nucleoplasm. hnRNP L has a molecular mass of 68 kDa and like hnRNP I, is also localized in the nucleoplasm.
• Synonyms: HNRPL, P/OKcl.14, Heterogeneous nuclear ribonucleoprotein L

Reference Data