Fas Ligand (FASLG) Rabbit Polyclonal Antibody

SKU AP06117PU-N

Fas Ligand (FASLG) rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: IF, IHC, WB
• Recommended Dilution:

  Western blot: 1/500-1/1000.; Immunohistochemistry on Paraffin sections: 1/50-1/200.; Immunofluorescence: 1/50-1/200.

• Reactivity: Human, Mouse
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human FasL.
• Specificity: This antibody detects endogenous levels of CD178 / Fas ligand protein (region surrounding Pro137).
• Buffer: Phosphate buffered saline (PBS), pH 7.2.; State: Aff - Purified; State: Liquid purified Ig fraction; Preservative: 0.05% Sodium azide
• Concentration: <span>1.0 mg/ml</span>
• Purification: Affinity chromatography using epitope-specific immunogen (> 95% pure by SDS-PAGE).
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Shipping: Blue Ice
• Predicted Protein Size: ~31 kDa
• Gene Name: Fas ligand
• Database Link:
  P48023
• Background: Cytotoxic T lymphocyte (CTL)-mediated cytotoxicity constitutes an important component of specific effector mechanisms in immuno-surveillance against virus-infected or transformed cells. Two mechanisms appear to account for this activity, one of which is the Perforin-based process. Independently, a FASbased mechanism involves the transducing molecule FAS (also designated Apo-1) and its ligand (FAS-L). The human FAS protein is a cell surface glycoprotein that belongs to a family of receptors that includes CD40, nerve growth factor receptors and tumor necrosis factor receptors. The FAS antigen is expressed on a broad range of lymphoid cell lines, certain of which undergo apoptosis in response to treatment with antibody to FAS. These findings strongly imply that targeted cell death is potentially mediated by the intercellular interactions of FAS with its ligand or effectors, and that FAS may be critically involved in CTL-mediated cytotoxicity.
• Synonyms: FASLG, APT1LG1, FASL, TNFSF6, CD95L protein, APTL

Reference Data