Cyclin E1 (CCNE1) Rabbit Polyclonal Antibody

SKU AP06082PU-N

Cyclin E1 (CCNE1) rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: ELISA, IF, IHC, WB
• Recommended Dilution:

  Western blot: 1/500-1/1000.; Immunohistochemistry on Paraffin sections: 1/50-1/200.; Immunofluorescence: 1/50-1/200.

• Reactivity: Human, Mouse
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Cyclin E1 protein (region surrounding Leu389).
• Buffer: Phosphate buffered saline (PBS), pH 7.2.; State: Aff - Purified; State: Liquid purified Ig fraction; Preservative: 0.05% Sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography using epitope-specific immunogen (> 95% pure by SDS-PAGE)
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Shipping: Blue Ice
• Predicted Protein Size: ~48 kDa
• Gene Name: cyclin E1
• Database Link:
  P24864
• Background: Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner. The cyclin E/E2F complex is detected primarily during the G1 phase of the cell cycle and decreases as cells enter S phase. E2F is known to be a critical transcription factor for expression of several S phase specific proteins.
• Synonyms: Cyclin-E1, CCNE1, CCNE

Reference Data