Acetylated Lysine Rabbit Polyclonal Antibody

SKU AP03035BT-N

Acetylated Lysine rabbit polyclonal antibody, Biotin

Product Data

• Application: ELISA, IF, IP, WB
• Recommended Dilution: ELISA. ; Western blot: A 1/250 dilution of this Biotin conjugated antibody AP03035BT-N was sufficient to detect the acetylated histone from TSA treated mouse spleen cell in western blot analysis (Ref.6).; Immunofluorescence. ; Immunoprecipitation (Ref.7)
• Antibody Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Acetylated KLH
• Specificity: This antibody detects proteins containing Acetylated Lysine residues in SDS-PAGE immunoblots. ; No reaction to Non-Acetylated proteins.
• Buffer: PBS; Label: Biotin; State: Liquid purified Ig fraction; Stabilizer: 50% Glycerol; Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Biotin
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Shipping: Blue Ice
• Background: Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves acetylation (1).; The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2).; Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3).; The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anticancer drugs currently in development (5).
• Synonyms: AcK, acetyl Lysine, acetyl-Lysine

Reference Data