Cytokeratin 18 (KRT18) Rabbit Polyclonal Antibody
Product Data | |
Application | IHC, WB |
---|---|
Recommended Dilution | Western blot: 1/500-1/1000. Note: Incubate membrane with diluted antibody in 5% nonfat milk, 1xTBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry on Paraffin Sections: 1/50-1/100. |
Reactivity | Human |
Antibody Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Peptide Sequenece around amino acids 31~35 (A-A-S-V-Y) derived from Human Keratin 18. |
Specificity | This antibody detects endogenous levels of total Keratin 18 protein. |
Buffer | PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol. State: Aff - Purified State: Liquid purified IgG fraction. |
Concentration | lot specific |
Purification | Affinity-Chromatography using epitope-specific immunogen. |
Conjugation | Unconjugated |
Storage | Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
Stability | Shelf life: One year from despatch. |
Shipping | Blue Ice |
Gene Name | keratin 18 |
Database Link | |
Background | Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18. |
Synonyms | Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18 |
Note | Molecular Weight: 46 kDa |
Reference Data |
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