ELK1 Rabbit Polyclonal Antibody

SKU AP02579PU-S

ELK1 rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: IF, IHC, WB
• Recommended Dilution: Immunohistochemistry on paraffin sections: 1/50 - 1/100.; Immunofluorescence: 1/100 - 1/200.; Western Blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
• Reactivity: Human, Mouse
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic non-phosphopeptide derived from human Elk-1 around the phosphorylation site of serine 389 (P-R-SP-P-A).
• Specificity: Elk-1 antibody detects endogenous levels of total Elk-1 protein.
• Buffer: PBS(without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol; State: Aff - Purified; State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: ELK1, ETS transcription factor
• Database Link:
  P19419
• Background: The transcription factor Elk1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors. Elk1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C terminus. Phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk1. Elk1 appears to be a direct target of activated MAP kinase. Biochemical studies indicate that Elk1 is a good substrate for MAP kinase, the kinetics of Elk1 phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block Elk1 activation in vivo. More recent studies have shown that Elk1 (Ser383) is also a target of the Stress Activated Kinase SAPK/JNK. Phosphorylation of Elk1 has also been implicated in synaptic plasticity in the adult hippocampus.
• Synonyms: Elk-1

Reference Data