GSK3 beta (GSK3B) pTyr216 Rabbit Polyclonal Antibody

SKU AP02535PU-N

GSK3 beta (GSK3B) pTyr216 rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500~1/1000.; Incubate membrane with diluted antibody in 5% nonfat milk, 1xTBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.; Immunofluorescence: 1/100-1/200.; Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
• Reactivity: Human, Mouse, Rat
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of tyrosine 279/216 (V-S-Y(p)-I-C) derived from Human GSK3α/β.
• Specificity: The antibody detects endogenous level of GSK3α/β only when phosphorylated at tyrosine 279/216.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol.; State: Aff - Purified; State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: glycogen synthase kinase 3 beta
• Database Link:
  P49841
• Background: Glycogen Synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. GSK3 has been implicated in fundamental cell processes such as cell fate determination, metabolism, transcriptional control and oncogenesis. Two isoforms, alpha (GSK3A; OMIM 606784) and beta (GSK3B) show a high degree of amino acid homology within their catalytic domains. GSK3B is involved in energy metabolism, neuronal cell development and body pattern formation.
• Synonyms: GSK-3 beta, Glycogen synthase kinase-3 beta, GSK3B
• Note: Molecular Weight: 46 kDa, 51 kDa

Reference Data