PKC theta (PRKCQ) pSer676 Rabbit Polyclonal Antibody

SKU AP02534PU-S

PKC theta (PRKCQ) pSer676 rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.; Immunofluorescence: 1/100-1/200.; Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
• Reactivity: Human, Mouse, Rat
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human PKCθ around the phosphorylation site of serine 676 (R-L-SP-F-A).
• Specificity: This antibody detects endogenous levels of PKC-theta only when phosphorylated at Serine 676.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.; State: Aff - Purified; State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.; Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Shipping: Blue Ice
• Gene Name: protein kinase C theta
• Database Link:
  Q04759
• Background: Protein kinase C (PKC) is a family of serine/threonine kinases that plays crucial roles in signal transduction. Members of the PKC family are divided into three groups based on their molecular structures and activating mechanisms: 1) Conventional PKC (alpha, beta, and gamma) requiring calcium, phosphatidylserine (PS), and diacylglycerol (DG) for activation; 2) novel PKC (sigma, epsilon, eta, and theta) activated independent of calcium; and 3) Atypical PKC (zeta and lamda), which are independent of both calcium and DG. High-level expression of PKC-theta was found in skeletal muscle, lung, T cells, and brain, and minimal expression in cardiac muscle, placenta, and liver. PKC theta is a autophosphorylated protein kinase.
• Synonyms: PKC theta, PKC-theta, nPKC-theta, Protein kinase C theta type

Reference Data