Cortactin pTyr466 Rabbit Polyclonal Antibody

SKU AP02517PU-N

Cortactin pTyr466 rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: WB
• Recommended Dilution: Western Blot: 1:500~1:1000.
• Reactivity: Mouse
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from mouse Cortactin around the phosphorylation site of tyrosine 466 (P-V-YP-E-T).
• Specificity: Cortactin antibody detects endogenous levels of Cortactin only when phosphorylated at tyrosine 466.
• Buffer: PBS(without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol; State: Aff - Purified; State: Liquid purified IgG
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: cortactin
• Database Link:
  Q60598
• Background: Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino terminal tandem repeats of a unique 37 amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha helical structure followed by a proline rich region. Between the proline rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr421, Tyr470, and Tyr486). In vitro, cortactin binds to and cross links F actin into meshworks.
• Synonyms: CTTN, EMS1, Amplaxin, Oncogene EMS1

Reference Data