HSP27 (HSPB1) pSer15 Rabbit Polyclonal Antibody

SKU AP02446PU-N

HSP27 (HSPB1) pSer15 rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.; Immunohistochemistry on paraffin-embedded sections: 1/50 - 1/100.; Immunofluorescence: 1/100 - 1/200.
• Reactivity: Human
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human HSP27 around the phosphorylation site of serine15 (G-P-SP-W-D).
• Specificity: HSP27 (phospho-Ser15) antibody detects endogenous levels of HSP27only when phosphorylated at serine 15.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.; State: Aff - Purified; State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity chromatography using epitope-specific phosphopeptide.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.; Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Shipping: Blue Ice
• Gene Name: heat shock protein family B (small) member 1
• Database Link:
  P04792
• Background: Hsp27, also referred to as the Estrogen regulated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. Hsp27 synthesis is induced by elevated temperature, as well as estrogen in hormone responsive cells. This protein is involved in stress resistance and actin organization. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSP's and mammalian a-crystalline lens protein. Because of the estrogen responsive nature of Hsp27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. This work has led to the suggestion that Hsp27 may be a useful marker in classifying various hormone sensitive tumors.
• Synonyms: Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein, SRP27, HSP25

Reference Data