Her2 (ERBB2) pTyr1248 Rabbit Polyclonal Antibody

SKU AP02377PU-S

Her2 (ERBB2) pTyr1248 rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.; Immunofluorescence: 1/100-1/200. ; Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivity: Human, Mouse, Rat
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from Human HER2 around the phosphorylation site of Tyrosine 1248 (P-E-Yp-L-G).
• Specificity: This antibody detects endogenous levels of HER2 only when phosphorylated at Tyrosine1248.
• Buffer: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl; State: Aff - Purified; State: Liquid purified Ig fraction; Preservative: no BSA/AMINES, and no glycerol.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Predicted Protein Size: 185 kDa (Predicted)
• Gene Name: erb-b2 receptor tyrosine kinase 2
• Database Link:
  P04626
• Background: ErbB 2 is a receptor tyrosine kinase of the ErbB 2 family. It is closely related instructure to the epidermal growth factor receptor. ErbB 2 oncoprotein is detectable in a proportion of breast and other adenocarconomas, as well as transitional cell carcinomas. In the case of breast cancer, expression determined by immunohistochemistry has been shown to be associated with poor prognosis.
• Synonyms: HER-2, NEU, p185erbB2, NGL, c-erbB-2, MNL19

Reference Data