ARFIP1 Rabbit Polyclonal Antibody

SKU AP01383PU-N

ARFIP1 rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.; Immunofluorescence: 1/50-1/200.; Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivity: Human, Mouse, Rat
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 300-350 of Human Arfaptin 1.
• Specificity: This antibody detects endogenous levels of Arfaptin-1 protein. (region surrounding Lys314)
• Buffer: Phosphate buffered saline (PBS), pH~7.2; State: Aff - Purified; State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE); Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Predicted Protein Size: ~40 kDa
• Gene Name: ADP ribosylation factor interacting protein 1
• Database Link:
  P53367
• Background: Arfaptin 1 is an ARF binding protein that inhibits PLD activation, vesicular trafficking and secretion. In the present report, we show that arfaptin 1 interacts with 'high speed' membranes independently of ARF. However, addition of myristoylated ARF3 (myrARF3) increases the association of arfaptin 1 with the membranes, suggesting that arfaptin 1 and ARF form a complex on the Golgi. Utilizing several deletion mutants of arfaptin 1 it is shown that the association of arfaptin 1 with myrARF3 is mediated via two binding sites on arfaptin 1. These two domains are needed for arfaptin 1 inhibition of PLD activation by myrARF3 in vitro.
• Synonyms: ARFIP1

Reference Data