Smad Interacting Protein 1 (ZEB2) Rabbit Polyclonal Antibody

SKU AP01369PU-N

Smad Interacting Protein 1 (ZEB2) rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000. ; Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivity: Human, Mouse
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 71-120 of Human SIP1.
• Specificity: This antibody detects endogenous levels of SIP1 protein. (region surrounding Glu101)
• Buffer: Phosphate buffered saline (PBS), pH~7.2; State: Aff - Purified; State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE); Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Predicted Protein Size: ~157 kDa
• Gene Name: zinc finger E-box binding homeobox 2
• Database Link:
  O60315
• Background: SMAD regulates gene expression by interacting with different classes of transcription factors including DNA-binding multi-zinc finger proteins. SIP1, for SMAD interacting protein 1, is a member of the delta-EF1/Zfh1 family of 2-handed zinc finger/homeodomain proteins. SIP1 contains a SMAD-binding domain, a homeodomain and two clusters of zinc fingers on the N- and C-termini. SIP1, also known as SMADIP1 and ZFHX1B, can be induced by TGF beta treatment. SIP1 plays a crucial role in normal embryonic development of neural structures and the neural crest. The human SIP1 gene maps to chromosome 2q22. Mutations in the SIP1 gene cause a form of Hirschsprung disease (HSCR). Patients with SIP1 mutations show mental retardation, delayed motor development, epilepsy, microcephaly, distinct facial features and/or congenital heart disease-all symptoms of HSCR.
• Synonyms: ZFX1B, ZEB2, SMADIP1, SIP1, KIAA0569

Reference Data

• Protein Families: Druggable Genome, Transcription Factors