Neurofibromin (NF1) Rabbit Polyclonal Antibody

SKU AP01247PU-N

Neurofibromin (NF1) rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: WB
• Recommended Dilution: Western Blot: 1/500-1/1000.; Immunofluorescence: 1/50-1/200.
• Reactivity: Human, Mouse, Rat
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 1550-1600 of Human Neurofibromin.
• Specificity: This antibody detects endogenous levels of Neurofibromin protein. (region surrounding Arg1575)
• Buffer: Phosphate buffered saline (PBS), pH~7.2; State: Aff - Purified; State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).; Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Shipping: Blue Ice
• Predicted Protein Size: ~ 319 kDa
• Gene Name: neurofibromin 1
• Database Link:
  P21359
• Background: Neurofibromatosis type 1 (NF1), or von Reckinghausen neurofibromatosis, is one of the most common autosomal dominant disorders in humans. Early linkage analysis mapped the NF1 gene to chromosome 17. The predicted NF1 transcript encodes a 2818 amino acid protein designated NF1GRP. By sequence analysis, similarity has been demonstrated within a small region of NF1GRP and members of the Ras GAP gene family. Functionally, NF1GRP was shown by biochemical analysis involving RAS-GAP hydrolysis and functional complementation in yeast to further resemble GAP protein. The NF1 protein is expressed at relatively constant levels in a broad range of cell lines and tissues including brain, lung, liver, kidney, spleen, muscle and colon. Although little is known regarding the function of NF1GRP, the homology with the catalytic domain of proteins with GTPase activity suggests that the NF1GRP may also interact in vivo with Ras proteins.
• Synonyms: NF1,NF-1

Reference Data