ICAM3 Mouse Monoclonal Antibody [Clone ID: 186-2G9]

SKU AM50238PU-S

ICAM3 mouse monoclonal antibody, clone 186-2G9, Purified

Product Data

• Clone Name: 186-2G9
• Application: FC, IF, IHC, IP, WB
• Recommended Dilution: Functional Studies: Blocks binding of CD11a (LFA-1) to CD50 (Use Azide free Antibody). ; ELISA: Use BSA free Antibody for Coating.; Western Blot: 0.5-1 μg/ml.; Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.; Flow Cytometry: 0.5-1 μg/10⁶ cells.; Immunofluorescence: 0.5-1 μg/ml.; Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.; Positive Control: HL-60 or THP-1 cells. Lymph node and tonsil.
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Stimulated Human leukocytes.
• Specificity: Recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (Leucocyte Workshop VI). CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This Monoclonal Antibopdy is excellent for staining of formalin/paraffin tissues.; Cellular Localization: Cell surface.
• Buffer: 10mM PBS; State: Purified; State: Liquid purified IgG fraction from Bioreactor Concentrate; Stabilizer: 0.05% BSA; Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Predicted Protein Size: 110-160 kDa
• Gene Name: intercellular adhesion molecule 3
• Database Link:
  P32942
• Synonyms: ICAM-3, ICAM-R, CDw50

Reference Data