Nkx2.2 (NKX2-2) Mouse Monoclonal Antibody [Clone ID: SPM564]

SKU AM50148PU-S

Nkx2.2 (NKX2-2) mouse monoclonal antibody, clone SPM564, Purified

Product Data

• Clone Name: SPM564
• Application: FC, IF, IHC
• Recommended Dilution: Flow Cytometry: 0.5-1 μg/million cells.; Immunofluorescence: 1-2 μg/ml.; Immunohistochemistry on Formalin-Fixed Paraffin Sections: 0.5-1.0 μg/ml for 30 minutes at RT.; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.; Positive Control: Pancreas or Ewing sarcoma.
• Reactivity: Chicken, Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human NKX2.2 recombinant protein.
• Specificity: Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. It acts as a valuable marker for Ewing sarcoma, with a sensitivity of 93% and a specificity of 89%, and aids in the differential diagnosis of small round cell tumors.; Cellular Localization: Nuclear.
• Buffer: 10mM PBS; State: Purified; State: Liquid purified IgG fraction from Bioreactor Concentrate; Stabilizer: 0.05% BSA; Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Predicted Protein Size: 40-50 kDa
• Gene Name: NK2 homeobox 2
• Database Link:
  O95096
• Synonyms: NKX2.2, NKX2B

Reference Data