Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody [Clone ID: SPM265]
| Product Data | |
| Clone Name | SPM265 |
|---|---|
| Application | FC, IF, IHC, WB |
| Recommended Dilution | Flow Cytometry: 0.5-1 μg/million cells. Immunofluorescence: 1-2 μg/ml. Western Blotting: 0.5-1 μg/ml. Immunoprecipitation: 1-2 μg/500 μg protein lysate. Immunohistochemistry on Formalin-Fixed Paraffin Sections: 0.5-1.0 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: MCF-7, HeLa, A431 cells or Breast Cancer. |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | PMC-42 human breast carcinoma cells. |
| Specificity | This Monoclonal Antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Cellular Localization: Cytoplasmic. Negative Species: Mouse, Rat, Sheep, Hamster, Cow, Dog and Pig. |
| Buffer | 10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide |
| Concentration | lot specific |
| Purification | Protein A/G Chromatography |
| Conjugation | Unconjugated |
| Storage | Store undiluted at 2-8°C. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Predicted Protein Size | 45 kDa |
| Gene Name | keratin 18 |
| Database Link | |
| Synonyms | Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18 |
| Reference Data | |
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