ECM1 Mouse Monoclonal Antibody [Clone ID: SPM217]

SKU AM50106PU-S

ECM1 mouse monoclonal antibody, clone SPM217, Purified

Product Data

• Clone Name: SPM217
• Application: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use Antibody without BSA for Coating.; Flow Cytometry: 0.5-1 μg/10⁶ cells.; Immunofluorescence: 1-2 μg/ml.; Western Blot: 0.5-1 μg/ml.; Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.; Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.; Positive Control: Stomach, Lung, or breast tumor.
• Reactivity: Human, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Secretory Component protein isolated from human colostrum.
• Specificity: This Monoclonal Antibody reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. ; The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.; Cellular Localization: Cell surface and cytoplasmic.
• Buffer: 10mM PBS; State: Purified; State: Liquid purified IgG fraction from Bioreactor Concentrate; Stabilizer: 0.05% BSA; Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Predicted Protein Size: ~80 kDa
• Gene Name: extracellular matrix protein 1
• Database Link:
  Q16610
• Synonyms: Secretory component p85, Extracellular matrix protein 1

Reference Data