Thymine DNA glycosylase (TDG) Mouse Monoclonal Antibody [Clone ID: AT2F7]

SKU AM50036PU-S

Thymine DNA glycosylase (TDG) mouse monoclonal antibody, clone AT2F7, Purified

Product Data

• Clone Name: AT2F7
• Application: ELISA, WB
• Recommended Dilution: The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:250.
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human TDG (1-410aa) purified from E. coli
• Buffer: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol; State: Purified; State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: thymine DNA glycosylase
• Database Link:
  Q13569
• Background: G/T mismatch-specific thymine DNA glycosylase is an enzyme that in humans is encoded by the TDG gene. Several bacterial proteins have strong sequence homology with this protein. The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12.
• Synonyms: E130317C12Rik; hTDG; JZA-3; Jza1; OTTMUSP00000028912; OTTMUSP00000028913

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair