ACAT1 Mouse Monoclonal Antibody [Clone ID: AT15E5]

SKU AM50027PU-S

ACAT1 mouse monoclonal antibody, clone AT15E5, Purified

Product Data

• Clone Name: AT15E5
• Application: ELISA, WB
• Recommended Dilution: The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1/1000.
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human ACAT1 (34-427aa) purified from E. coli
• Buffer: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol; State: Purified; State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: acetyl-CoA acetyltransferase 1
• Database Link:
  P24752
• Background: ACAT1 (acetyl-Coenzyme A acetyltransferase 1) is a 417 amino acid protein. ACAT1 is a mitochondrial enzyme involved in the formation and degradation of ketone bodies and is necessary for the proper metabolic processing of isoleucine. ACAT1 and ACAT2 catalyze the formation of acetoacetyl-CoA from two acetyl-CoA molecules. These enzymes are also capable of the reverse reaction. Defects in ACAT1 are a cause of 3-ketothiolase deficiency. 3-ketothiolase deficiency is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded.
• Synonyms: ACAT; MAT; T2; THIL

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Butanoate metabolism, Fatty acid metabolism, leucine and isoleucine degradation, Lysine degradation, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism, Synthesis and degradation of ketone bodies, Terpenoid backbone biosynthesis, Tryptophan metabolism, Valine