Biotin Mouse Monoclonal Antibody [Clone ID: Hyb-8]

SKU AM33308PU-T

Biotin mouse monoclonal antibody, clone Hyb-8, Purified

Product Data

• Clone Name: Hyb-8
• Application: FC, IF, IHC, WB
• Recommended Dilution: Western Blotting.; Confocal Microscopy. ; Flow Cytometry: 0.5-1 µg/10⁶ cells. ; In situ Nucleic Acid Hybridization. ; Immunofluorescence: 0.5-1 µg/ml. ; Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1 µg/ml for 30 minutes at RT. ; No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.; Recommended Positive Control: Biotinylated proteins in solution or on tissues.
• Reactivity: All Species
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Biotinylated Sheep immunoglobulin.
• Specificity: It recognizes both the free and protein-conjugated (either soluble or cell bound) form of Biotin. ; This Monoclonal Antibody is highly specific to Biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for Biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-Biotin antibody may be substituted to decrease this noise.; Cellular Localization: Depends on the target.
• Buffer: 10mM PBS; State: Purified; State: Liquid purified IgG fraction from Bioreactor Concentrate; Stabilizer: 0.05% BSA; Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.; DO NOT FREEZE!
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Predicted Protein Size: 244 kDa
• Synonyms: Vitamin B7, Vitamin H

Reference Data