CD63 (Late Endosomes Marker) Mouse Monoclonal Antibody [Clone ID: NKI/C3]

SKU AM33301PU-S

CD63 (Late Endosomes Marker) mouse monoclonal antibody, clone NKI/C3, Purified

Product Data

• Clone Name: NKI/C3
• Application: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA Free antibody for coating.; Flow Cytometry: 0.5-1 µg/10⁶ cells. ; Immunofluorescence: 0.5-1 µg/ml. ; Western Blotting: 0.5-1 µg/ml. ; Immunoprecipitation: 0.5-1 µg/500 µg protein lysate. ; Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT. ; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. ; Recommended Positive Control: SK-MEL-28, HL60, THP-1 or NIH/3T3 cells. Melanoma or lymphoma.
• Reactivity: Human, Mouse
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Smooth plasma membrane fraction of MeWo cells.
• Specificity: This Monoclonal antibody recognizes Human and Mouse CD63. Other species not tested. ; It recognizes a protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. ; Cellular Localization: Cytoplamsic.
• Buffer: 10mM PBS; State: Purified; State: Liquid purified IgG fraction from Bioreactor Concentrate; Stabilizer: 0.05% BSA; Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.; DO NOT FREEZE!
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Predicted Protein Size: 26 kDa (core protein); 30-60 kDa (glycosylated)
• Gene Name: CD63 molecule
• Database Link:
  P08962
• Background: The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
• Synonyms: OMA81H, Granulophysin, Tetraspanin-30, MLA1, TSPAN30, ME491

Reference Data