Dibromo-Tyrosine (DiBrY) Mouse Monoclonal Antibody [Clone ID: 3A5]

SKU AM33191PU-N

Dibromo-Tyrosine (DiBrY) mouse monoclonal antibody, clone 3A5, Purified

Product Data

• Clone Name: 3A5
• Application: ELISA, IHC, WB
• Recommended Dilution: ELISA (Ref.1).; Western blot. ; immunohistochemistry on Paraffin Sections: 10 μg/ml (Ref.1).
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: 3,5-Dibromo-Tyrosine KLH-conjugated.
• Specificity: Specific for di-halogenated Tyrosine. Recognizes Dibromo-Tyrosine (DiBrY), N-acetylated dichlorotyrosine (NAcDiClY), diiodo-tyrosine (DiIY), N-bromo-succinimide-treated BSA, HOCl-treated BSA and dibromo-tyrosine conjugated BSA. Bromotyrosine (BrY) is weakly recognized. ; There was no cross reactivity obtained with dityrosine-conjugated BSA, peroxynitrite-treated BSA, tyrosine (Y), chlorotyrosine(ClY), 3-3’-dityrosine (DiY), 3-aminotyrosine (NH2Y), 3-nitrotyrosine (NO2Y), phenylalanine (Phe), o-phosphotyrosine, 3, 4-dihydroxyphenylacetic acid (DOPA) and 2-hydroxy-4-phenylacetic acid. No cross reactivity was obtained with brominated nucleic acids 5-bromouracil, 5-bromouridine, and 8-bromoguanosine.
• Buffer: 10 mM PBS pH 7.4; State: Purified; State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore in 200 µl aqua bidest to 0.1 mg/ml
• Purification: Protein A Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.; After reconstitution store the antibody undiluted (in aliquots) at -20°C.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Ambient
• Synonyms: Diamine oxidase, AOC1, DAO1, Amiloride-binding protein, Histaminase, Kidney amine oxidase

Reference Data