ICAM3 Mouse Monoclonal Antibody [Clone ID: JA4]

CAT#: AM33037PU-N

ICAM3 mouse monoclonal antibody, clone JA4, Purified

Product Images

Jurkat T cells that expressed high (ICAM-3high= red) or low (ICAM-3low= blue) levels of ICAM-3 were stained with JA4. 5 x 105 cells were stained with 100ul of mAb. Following 30 min incubation at 4°C, unbound Ab was removed by washing and bound mAb detected by staining with goat antimouse- FITC for 30 min at 4°C. Flow cytometric histogram of 5000 events per sample is shown.

Specifications

Product Data

• Clone Name: JA4
• Applications: ELISA, FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA.
  Western Blot.
  Immunoprecipitation.
  Flow Cytometry.
  Immunocytochemistry.
  Immunohistochemistry on Frozen Sections.
  Recommended Dilutions: 1/100–1/200 for Flow Cytometry, and for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1/100–1/1000 for Immunoblotting applications.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Derived by fusion of mouse Ag8.653 cells with spleen cells from a BALB/c mouse immunized with an ICAM-3/HEK transfectant.
• Specificity: This JA4 antibody recognizes a linear epitope in domains 1 and 2 of ICAM-3.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: intercellular adhesion molecule 3
• Database Link:
  Entrez Gene 3385 Human
  UniProt ID P32942
• Background: Intercellular adhesion molecule 3 (ICAM3) also known as CD50 (Cluster of Differentiation 50), is a type I transmembrane glycoprotein, which normally contain 2-9 immunoglobulin-like C2-type domains, and binds to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM-3 has previously been implicated in apoptotic cell clearance, although its precise role in the clearance process is ill defined.
• Synonyms: ICAM-3, ICAM-R, CDw50