Thyroglobulin (TG) Mouse Monoclonal Antibody [Clone ID: 6E1]

SKU AM32841PU-T

Thyroglobulin (TG) mouse monoclonal antibody, clone 6E1, Purified

Product Data

• Clone Name: 6E1
• Application: FC, IHC, WB
• Recommended Dilution: ELISA (For coating, use Antibody without BSA). ; Western Blot: 0.5-1 µg/ml. ; Flow Cytometry: 0.5-1 µg/10⁶ cells. ; Immunohistochemistry on Paraffin Sections: 0.5-1 µg/ml for 30 min at RT.; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. ; Positive Control: Thyroid.
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Thyroglobulin isolated from Human thyroid glands.
• Specificity: This antibody is useful in identification of thyroid carcinoma of the papillary and follicular types. Presence of Thyroglobulin in metastatic lesions establishes the thyroid origin of tumor. ; Anti-Thyroglobulin, combined with anti-Calcitonin, can identify medullary carcinomas of the thyroid. Furthermore, anti-Thyroglobulin, combined with anti-TTF1, can be a reliable marker to differentiate between primary thyroid and lung neoplasms. ; Cellular Localization: Cytoplasmic, Secreted.
• Buffer: 10mM PBS; State: Purified; State: Liquid purified IgG fraction from Bioreactor Concentrate; Stabilizer: 0.05% BSA; Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Predicted Protein Size: 660 kDa (Dimeric Form)
• Gene Name: thyroglobulin
• Database Link:
  P01266
• Background: Thyroglobulin is a 660 kDa dimeric preprotein with mutiple glycosylation sites is produced by and processed within the thyroid gland to produce the hormone thyroxine and triiodothyronine. Prior to forming dimers, thyroglobulin monomers undergo conformation maturation in the endoplasmic reticulation. Thyroglobulin dimerization as well as transport of thyroglobulin to the Golgi complex is calcium dependent. Thyroglobulin defects resulting from defective dimer formation and export to the Golgi is thought to cause some types of goiter. Antibody against thryoglobulin may be produced by individuals with other diseases arising from the gland such as Hashimoto's or Graves disease. Hence the presence of thyroglobulin autoantibodies can help to identify disease. Antibody to thyroglobulin has been shown to be useful for the identification of papillarly and follicular thyroid carcinoma; thyroglobulin antibody positive lesions are of thyroidal origin. Carcinomas of nonthyroidal origin do not express thyroglobulin and hence are thyroglobulin antibody negative. It is important to note though that not every type of thyroidal lesion is thyroglobulin antibody positive, a number of forms are negative. Hence a negative result does not necessarily rule out that a given lesion or metastasis originated from the thyroid gland.
• Synonyms: Thyreoglobulin, TGN

Reference Data