CD34 Mouse Monoclonal Antibody [Clone ID: EQ-8D11-C1]
Product Data | |
Clone Name | EQ-8D11-C1 |
---|---|
Application | ELISA, FC, IF, WB |
Recommended Dilution | Flow Cytometry: 1.2 μg/106 cells. Immunofluorescence: 1 μg/106 cells. Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400. |
Reactivity | Sheep |
Antibody Host | Mouse |
Isotype | IgG2a |
Clonality | Monoclonal |
Immunogen | Genetic immunisation with cDNA encoding the extracellular domain of Ovine CD34 |
Specificity | This antibody recognizes Ovine CD34. Based on recognition of the complete native protein expressed on transfected mammalian cells. Other species not tested. Cellular Localization: Membrane. |
Buffer | PBS, pH 7.2 State: Purified State: Liquid purified Ig fraction Preservative: None |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein G |
Conjugation | Unconjugated |
Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. |
Stability | Shelf life: one year from despatch. |
Shipping | Blue Ice |
Background | CD34 is a type I membrane protein with an apparent MW of approximately 115 kDa. It is a putative adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. CD34 is expressed specifically on immature normal human marrow cells, including hematopoietic progenitor cells (2). It is thought to act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. |
Synonyms | Hematopoietic progenitor cell marker |
Reference Data |
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