Egf Mouse Monoclonal Antibody [Clone ID: F5]

SKU AM32085SU-N

Egf mouse monoclonal antibody, clone F5, Supernatant

Product Data

• Clone Name: F5
• Application: ELISA, IHC, WB
• Recommended Dilution: ELISA. ; Spot Blots. ; Immunohistochemistry on Fixed Frozen Sections: 1/20. ; Immunohistochemistry on Paraffin Sections of Salivary glands (see Protocols for more details.)
• Reactivity: Mouse
• Antibody Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: EGF coupled on BSA and tetanus toxoide
• Specificity: The antibody reacts with Mouse EGF in ELISA (10 ng detectable) and in Spot Blots (1 ng detectable). ; In Immunohistochemistry the antibody reacts with formalin fixed and paraffin embedded Mouse salivary glands. ; It also reacts with Human Brunner's glands (presumably with urogastron).
• Buffer: State: Supernatant; State: Tissue Culture Supernatant; Stabilizer: 1.0% BSA; Preservative: 20 mM Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. ; Avoid repeated freezing and thawing. ;
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: epidermal growth factor
• Database Link:
  P01132
• Background: Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein. ; Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development.
• Synonyms: Urogastrone, Epidermal growth factor, URG, HOMG4

Reference Data