alpha 1 Spectrin (SPTA1) Mouse Monoclonal Antibody [Clone ID: AF10]

CAT#: AM32073PU-N

alpha 1 Spectrin (SPTA1) mouse monoclonal antibody, clone AF10, Purified

Specifications

Product Data

• Clone Name: AF10
• Applications: IF, IHC, IP, WB
• Recommended Dilution: Western blotting: at least 1/1000 dilution.
  Immunoprecipitation.
  Immunocytochemistry on Frozen Sections.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Ghost proteins of Human red blood cells.
  Hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.
• Specificity: The antibody clone AF10 is specific to the 240kD Erythroid alpha-Spectrin.
  This antibody is suitable for Various immunochemical reactions to detect Erythroid Spectrins, Identification of Erythroid leukemias (Ref.1).
• Formulation: PBS
  State: Purified
  State: Liquid purified Ig fraction
  Stabilizer: 1.0% BSA
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C.

• Stability: Shelf life: one year from despatch.

• Gene Name: spectrin alpha, erythrocytic 1
• Database Link:
  Entrez Gene 6708 Human
  UniProt ID P02549
• Background: Erythroid spectrins, some other proteins of erythroid cytoskeleton, and the transmembrane protein band 3 are highly specific for erythrocytes and their progenitors. They are more reliable markers for erythroid differentiation than Glycophorin A, the commonly used marker for erythroid differentiation, because Glycophorin A is expressed also in many cell lines otherwise exhibiting mainly megakaryotic charasteristics.
• Synonyms: SPTA, Erythroid alpha-spectrin