alpha 1 Spectrin (SPTA1) Mouse Monoclonal Antibody [Clone ID: AF10]
| Product Data | |
| Clone Name | AF10 |
|---|---|
| Application | IF, IHC, IP, WB |
| Recommended Dilution | Western blotting: at least 1/1000 dilution. Immunoprecipitation. Immunocytochemistry on Frozen Sections. |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Ghost proteins of Human red blood cells. Hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. |
| Specificity | The antibody clone AF10 is specific to the 240kD Erythroid alpha-Spectrin. This antibody is suitable for Various immunochemical reactions to detect Erythroid Spectrins, Identification of Erythroid leukemias (Ref.1). |
| Buffer | PBS State: Purified State: Liquid purified Ig fraction Stabilizer: 1.0% BSA Preservative: 0.09% Sodium Azide |
| Concentration | lot specific |
| Conjugation | Unconjugated |
| Storage | Store the antibody undiluted at 2-8°C. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | spectrin alpha, erythrocytic 1 |
| Database Link | |
| Background | Erythroid spectrins, some other proteins of erythroid cytoskeleton, and the transmembrane protein band 3 are highly specific for erythrocytes and their progenitors. They are more reliable markers for erythroid differentiation than Glycophorin A, the commonly used marker for erythroid differentiation, because Glycophorin A is expressed also in many cell lines otherwise exhibiting mainly megakaryotic charasteristics. |
| Synonyms | SPTA, Erythroid alpha-spectrin |
| Reference Data | |
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