TCR DO-11.10 Mouse Monoclonal Antibody [Clone ID: KJ1-26]

SKU AM31863PU-N

TCR DO-11.10 mouse monoclonal antibody, clone KJ1-26, Purified

Product Data

• Clone Name: KJ1-26
• Application: FC
• Recommended Dilution: Flow Cytometry (See Protocols).; This clone has also been reported to work in Immunoprecipitation, ELISA and Inhibition Assay.
• Reactivity: Mouse
• Antibody Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: T cell hybridoma, D011.10.; Donor: Mouse ascites fluid.
• Specificity: This Monoclonal Antibody binds the T-cell receptor (TCR) expressed on DO11.10 and DO11.10.24 murine T cell hybridomas, but not on any other T cell hybridomas tested. (1); The TCR expressed on DO11.10 recognizes an ovalbumin peptide in the context of I-Ad major histocompatibility molecules. (1,2) The use of DO11.10 and Clone KJ1-26 have proven to be instrumental in describing many of the intricate details of T cell immunity. (3-6); The DO11.10 T cell hybridoma is available from the laboratories of Drs. Kappler and Marrack. (2,7)
• Buffer: PBS containing 0.02% Sodium Azide as preservative.; State: Purified; State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Protein G Affinity Chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Synonyms: DO-11.10 T cell Receptor
• Note: Protocol: Test Results (See Figure 1.); Tissue Distribution by Flow Cytometry Analysis:; Mouse Strain: BALB/c-[Tg] D011.10 TCR-[KO] RAG2.; Cell Concentration : 1x10e6 cells per tests.; Antibody Concentration Used: 0.5 µg/10e6 cells.; Isotypic Control (Shaded): Purified Mouse IgG2a.

Reference Data