CD30 (TNFRSF8) Mouse Monoclonal Antibody [Clone ID: Ber-H2]

CAT#: AM20190FC-N

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CD30 (TNFRSF8) mouse monoclonal antibody, clone Ber-H2, FITC



Size
    • 50 ug


Product images

Specifications

Product Data
Clone Name Ber-H2
Applications FC
Recommended Dilution Flow Cytometry: 5-10 µg/mL (final concentration).
Detailed procedure is provided in Protocols.
Reactivity Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Co cell line cells
Specificity This antibody reacts with CD30 on Flow Cytometry.
Formulation PBS containing 5% BSA and 0.09% Sodium Azide
Label: FITC
State: Liquid purified IgG fraction.
Concentration 50 µg/ml
Purification Protein-A Agarose Chromatography of hybridoma supernatant.
Conjugation FITC
Storage Store the antibody undiluted at 2-8°C.
Stability Shelf life: one year from despatch.
Gene Name Homo sapiens tumor necrosis factor receptor superfamily member 8 (TNFRSF8), transcript variant 1
Background CD30, also known as Ki-1, TNFRSF8, or Be-H2, is a 120 kDa glycoprotein expressed on the surface of mitogen-activated B-cells and T-cells but not on resting lymphocytes or monocytes. CD30 is also a marker for Hodgkin and Sternberg-Reed cells of Hodgkin's lymphomas and related hematologic malignancies. Soluble forms of CD30 have been found in the serum of patients with adult T-cell leukemia or other CD30+ lymphomas. The CD30 ligand, CD153, is a type II transmembrane glycoprotein that enhances proliferation of activated T-cells and induces apoptosis in CD30+ lymphoma-derived cell lines.
Synonyms TNFRSF8, D1S166E, CD30L receptor, KI-1 antigen
Note This product was originally produced by MBL International.

Protocol: Flow cytometric analysis for floating cells
We usually use Fisher tubes or equivalents as reaction tubes for all step described below.
1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and 0.1% NaN3].
2) Resuspend the cells with washing buffer (5x10e6 cells/mL).
3) Add 50 µL of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute at RT (20~25°C). Remove supernatant by careful aspiration.
4) Add 10 µL of normal goat serum containing 1 mg/mL normal human IgG and 0.1% NaN3 or 20 µL of Clear Back (human Fc receptor blocking reagent) to the cell pellet after tapping. Mix well and incubate for 5 minutes at room temperature.
5) Add 20 µL of the FITC labeled CD30 monoclonal antibody (Ber-H2) (5-10 µg/mL) diluted with the washing buffer. Mix well and incubate for 30 minutes at RT (20~25°C).
6) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at RT. Remove supernatant by careful aspiration.
7) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer.
Positive Control: CCRF-CEM
Flow cytometric analysis for whole blood cells
We usually use Falcon tubes or equivalents as reaction tubes for all steps described below.
1) Add 20 µL of the FITC labeled CD163 monoclonal antibody (Ber-Mac3) (5-10 µg/mL) diluted with the washing buffer [PBS containing 2% fetal calf serum
(FCS) and 0.1% NaN3] into each tube.
2) Add 50 µL of whole blood into each tube. Mix well, and incubate for 30 minutes at RT (20~25°C).
3) Lyse with OptiLyse C (for analysis on Beckman Coulter instruments) or OptiLyse B (for analysis on BD instruments), using the procedure recommended in the respective package inserts.
4) Add 1mL of H2O to each tube and incubate for 10 minutes at RT (20~25°).
5) Centrifuge at 500 x g for 1 minute at RT (20~25°C). Remove supernatant by careful aspiration.
6) Add 1 mL of washing buffer followed by centrifugation at 500 x g for 1 minute at RT (20~25°C). Remove supernatant by careful aspiration.
7) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer.
Reference Data
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transmembrane
Protein Pathways Cytokine-cytokine receptor interaction
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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