MLH1 Mouse Monoclonal Antibody [Clone ID: G168-728]
| Product Data | |
| Clone Name | G168-728 |
|---|---|
| Application | IHC |
| Recommended Dilution | Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/100 Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended. Use Polymer anti Mouse/Rabbit IgG as a detection system. Positive Control: Colon carcinoma. |
| Reactivity | Hamster, Human, Mouse, Rat |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Recombinant full length MLH protein. |
| Specificity | The G168-15 antibody recognizes the Human and Mouse MLH1 (80-85kDa). Cellular Localization: Nuclear. |
| Buffer | State: Supernatant State: Liquid Tissue Culture Supernatant with 0.2% BSA and 15mM Sodium Azide. |
| Conjugation | Unconjugated |
| Storage | Store the antibody undiluted at 2-8°C. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | mutL homolog 1 |
| Database Link | |
| Background | The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. The importance of mismatch repair genes became apparent with the identification of the genetic basis for hereditary nonpolyposis colon cancer (HNPC). MSH-2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMSH, which together help facilitate the later steps in mismatch repair. |
| Synonyms | DNA mismatch repair protein Mlh1, COCA2 |
| Reference Data | |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Melanogenesis, Melanoma, Pathways in cancer |
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