PCNA Mouse Monoclonal Antibody [Clone ID: PC10]

SKU AM10035PU-S

PCNA mouse monoclonal antibody, clone PC10, Purified

Product Data

• Clone Name: PC10
• Application: IF, IHC, WB
• Recommended Dilution: Immunohistochemistry and Immunocytochemistry Frozen or Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections and cell smears).; For IHC dilute conc. antibody at 1/50-1/200, use streptavidin~biotin system or polymer system, incubate 30 minutes at room temperature.; FFPE tissue section requires antigen retriever (boiling tissue in 10 mM citrate, pH 6.0 for 20 mins, followed by cooling at RT for 20 mins).; Recommended Positive Control: Human tonsil, lymph node.; Western Blot: 1/100-1/400.
• Reactivity: Human, Insect, Mouse, Rat, Yeast
• Antibody Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant Rat PCNA protein.
• Specificity: This antibody is specific for PCNA. ; PC10 reacts specifically with a 36kD nuclear protein of the Proliferating Cell Nuclear Antigen (PCNA).; PCNA is an excellent marker of proliferative cells in routinely processed tissue sections.; Cellular Localization: Nuclear.
• Buffer: PBS, pH 7.4; State: Purified; State: Liquid purified IgG fraction; Stabilizer: 1% BSA; Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Shipping: Blue Ice
• Gene Name: proliferating cell nuclear antigen
• Database Link:
  P12004
• Background: Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase delta in eukaryotic cells. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. PCNA was originally identified as an antigen that is expressed in the nuclei of cells during the DNA synthesis phase of the cell cycle It is increased during late G1 phase and S phase of the cell cycle and declines during G2 and M phases.
• Synonyms: Cyclin

Reference Data