Bak (BAK1) Mouse Monoclonal Antibody [Clone ID: AT8B4]

CAT#: AM09369PU-S

Bak (BAK1) mouse monoclonal antibody, clone AT8B4, Purified

Product Images

Western blot analysis: Mouse lung lysates (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human BAK1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human BAK1 antibody (1:500) with Texas Red (Red).

Specifications

Product Data

• Clone Name: AT8B4
• Applications: ELISA, FC, IF, WB
• Recommended Dilution: ELISA.
  Western blot: 1/250-1/500.
  Flow Cytometry.
  Immunocytochemistry/Immunofluorescence.
  Immunofluorescence: 1/250-1/500.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant Human BAK1 (29-187aa) purified from E. coli
• Specificity: The antibody recognizes Human and Mouse BAK1. Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: BCL2 antagonist/killer 1
• Database Link:
  Entrez Gene 12018 MouseEntrez Gene 578 Human
  UniProt ID Q16611
• Background: BAK1 encodes an receptor-like kinase (RLK) with a putative extracellular domain, a single transmembrane domain, an intracellular-juxtamembrane domain, and a kinase domain. BAK1 expression is associated with the progression of Prostate cancer (CaP). BAK1 appears to function in distinct receptor-signaling complexes to integrate multiple microbe-associated molecular pattern (MAMP) perception into downstream-signaling events. BAK1 can serve multiple purposes to simultaneously modulate MAMP-receptor complexes, BR signaling, and cell death for the benefit of bacterial infection, life style, and fitness.
• Synonyms: BAK1, BAK, BCL2L7, Bcl2-L-7, CDN1

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transmembrane