KCTD15 Mouse Monoclonal Antibody [Clone ID: AT4C3]

CAT#: AM09364PU-S

KCTD15 mouse monoclonal antibody, clone AT4C3, Purified

Product Images

Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human KCTD15 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Western blot analysis: Cell lysates of HeLa (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human KCTD15 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Immunofluorescence of human HeLa cells stained with monoclonal anti-human KCDT15 antibody (1:500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).

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  Immunofluorescence of human HeLa cells stained with monoclonal anti-human KCTD15 antibody (1:500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).

Specifications

Product Data

• Clone Name: AT4C3
• Applications: ELISA, FC, IF, WB
• Recommended Dilution: ELISA.
  Western blot (1:250 - 1:1000).
  Flow Cytometry.
  Immunofluorescence (1:250 - 1:500).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG3
• Clonality: Monoclonal
• Immunogen: Recombinant human KCTD15 (1-234aa) purified from E. coli
• Specificity: The antibody recognizes human KCTD15. Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: potassium channel tetramerization domain containing 15
• Database Link:
  Entrez Gene 79047 Human
  UniProt ID Q96SI1
• Background: KCTD15 (Potassium channel tetramerisation domain containing 15, also known as BTB/POZ domain-containing protein KCTD15) is protein that in humans is encoded by the KCTD15 gene. KCTD15 is expressed at high level in brain and hypothalamus. The potassium channel KCTD15 was identified as a genetic loci associated with higher than normal body mass index (BMI) in humans along with genes such as GNPDA2, MTCH2, FTO, and TMEM18. Single nucleotide polymorphisms (SNPs) in non-diabetic and diabetic patients showed that FTO was most strongly associated with obesity while MTCH2 and GNPDA2 were still significantly associated with higher than normal BMI levels.
• Synonyms: KCTD15, KCTD-15, BTB/POZ domain-containing protein KCTD15

Reference Data

• Protein Families: Ion Channels: Other