NFAT2 (NFATC1) Mouse Monoclonal Antibody [Clone ID: AT1C3]
Specifications
| Product Data | |
| Clone Name | AT1C3 |
|---|---|
| Application | ELISA, FACS, FC, ICC/IF, IF, IHC, WB |
| Recommended Dilution | ELISA. Western blot (1/1,000-1/2,000). Flow Cytometry. Immunofluorescence. Immunohistochemistry on Paraffin Sections (10 µg/ml). This NFATC1 antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG2a, kappa |
| Clonality | Monoclonal |
| Immunogen | Recombinant human NFATc1 (428-716aa) purified from E. coli |
| Specificity | The antibody recognizes Human NFATc1. Other species not tested. |
| Buffer | Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol |
| Concentration | 1mg/ml (determined by BCA assay) |
| Purification | By protein-G affinity chromatography |
| Conjugation | Unconjugated |
| Storage | Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | nuclear factor of activated T-cells 1 |
| Database Link | |
| Background | Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 (NFATc1) is a component of the nuclear factor of activated T cells DNA-binding transcription complex which consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. This protein plays a main role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription and functions as a major molecular target for the immunosuppressive drugs such as cyclosporine A. NFATc1 is expressed in most human primary lymphocytes and mature human T- and B-cell neoplasms. |
| Synonyms | nuclear factor of activated T-cells, cytosolic component 1 isoform A, NF-ATC, NFATc, NFAT2 |
| Reference Data | |
| Protein Categories | Cytokines, Growth Factors, Intracellular Proteins, Transciption Factors |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Axon guidance, B cell receptor signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway |
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.